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文献和实验Monoclonal Antibody Production Protocol
. It is advisable to do this spin at the same time that the second wash of the spleen cells is done. The fusion The washed myeloma and spleen cells are pooled in 30ml of PBS (room temp.) and spun gently at 1000 rpm for 10 min. Aspirate the PBS and resuspend
to do this spin at the same time that the second wash of the spleen cells is done. The fusion The washed myeloma and spleen cells are pooled in 30ml of PBS (room temp.) and spun gently at 1000 rpm for 10 min. Aspirate
Polyclonal and Monoclonal Antibodies
Maintenance of the cells: Stock solutions: IMDM : Fetal Calf Serum- Transferrin: Iron saturated. 1000X stock = 1 mg/ml HT supps: 50X from Sigma H0137 ( Store at -20 C ). 2-Mercaptoethanol: 1000X stock (5 x 10 -2). (Store
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