赛默飞世尔Thermo Fisher 125 mL Medi

Cell Cultures

media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml

Procedures for In Vitro Production of Bovine Embryos

Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM

Cool growth competent E . coli

al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria