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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:L-510-GRD-B-Q
中文名称:
英文名称:LOCKING 1.5ML MCT GRADUATED BLUE 5M/CS
货期:热销品现货,其他请咨询




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文献和实验Basic procedures for bacteria culture
Protocol 1. Add the following reagents to a 0.5 ml microcentrifuge tube, in the order listed: sterile ddH2O q.s 10X kinase buffer 1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul
in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15
-Hanahan (1983) J. Mol. Biol. 166, 557-580. XL1Blue-MRF' - D(mcrA)182, D(mcrCB-hsdSMR-mrr)172,endA1, supE44, thi-1, recA, gyrA96, relA1, lac, l-, [F'proAB, lac IqZDM15, Tn10 (tetr)] -For plating or glycerol stocks, grow in LB with 20 mg/ml
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