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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:BG60E
中文名称:
英文名称:BENCHGUARD, EXTRA 0.60
货期:热销品现货,其他请咨询




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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
) DMEM/F12 (Invitrogen, Catalog # 12500-096 or equivalent) Materials: BG01V human embryonic stem cells Tissue culture dishes (60 mm; Fisher, Catalog # 08-772B, 100 mm; Fisher, Catalog # 08-772E, or equivalent) 15 mL conical
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
8. Add TdT (0.3 e.u./m L) and biotinylated dUTP in TdT buffer to cover the sections. 9. Incubate in humid atmosphere at 37°C for 60 min. 10. Stop the reaction by transferring the slides to TB buffer for 15 min at room temperature. 11. Wash
CTAB TECHNIQUE / Method / Sched
-like consistency, mix occasionally. 4. Incubate for 60 min at 60℃. 5. Add equal volume of chloroform/iso-amyl alcohol (24:1), Mix for about 3min, then transfer contents to narrow bore centrifuge tubes. Balance by adding extra chlor/iso. Spin 5,000rpm for 10min
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