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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
A quick RNA mini-prep for Neurospora mycelial cultures
. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora and, potentially, other filamentous fungi. Below, we describe the use
for 6 hr at 55°C. (C ) Chromatogram of a commercial sample of uridine. Conditions: RP‐HPLC analysis is performed using UV detection (254 nm) and a 5 µm Supelcosil LC‐18S column (25 cm × 4.6 mm) according to the following method: starting from 0.1 M
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