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- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- WB30254
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：WB30254
中文名称：
英文名称：WEIGHING BOAT, SQUARE, WHITE, MEDIUM 10
货期：热销品现货，其他请咨询
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文献和实验,dilute your primary antibody in BSA/PBST (15 ml is enough for one blot in a little clear--square--box,use 25 ml for 2 blots).Pour off wash,then immediately add the primary antibody.Incubate at room temp,60 minutes or overnight at 4℃.Pour off.7.Wash 4 X 10
dH2O to 1 liter. Autoclave. 1X PBS-T: 100 ml 10X PBS 1 g Tween-20 (it's a liquid, weigh in little boat) 900 ml dH20 makes 1 Liter (this keeps on shelf indefinitely) Blocking buffer: 2.5 g powder non-fat milk in 50 ml 1X PBS-T
like rectangular white plastic cup --should be full of ice). Precut 3MM filter paper to size (10 cm X 7.5 cm)--Need 2. Cut nitrocellulose (handle with gloves!) to 8.5 cm X 6 cm. When gel is finished, recover it from the apparatus and cut off wells
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