赛默飞世尔Thermo Fisher BTLTOP A-PE

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

NaCl, 5 mM MgCl2, 100 mM Tris, pH 9.5; 0.1% Tween 20; 1mM Levamisole. Levamisole is a potential inhibitor of endogenous phosphatases.- TTBS: 150mM NaCl, 50mM Tris-HCl pH 7.8, 0.1% BSA, 0.1% Tween-20 E. Slides and other materials - Carter's

In Situ HYBRIDIZATION TO TISSUE SECTIONS

Add 10U Pol I. Incubate 45' @ room temperature. Stop the reaction by adding 25ml 50mM EDTA (pH 8), on ice. Determine the % incorporation by TCA precipitation: -Add 1ml reaction mixture to 100ml 0.5 mg/ml carrier DNA in a 7ml

质谱样本制备技术资料

水和甲醇各涮一遍，真空干燥。 3. 胰酶储液制备：使胰酶粉末沉落瓶底，用100μ胰酶重旋液溶解，用0.5ml EP分装成5μl。 4. 50ml 100 mM NH4HCO3。 脱色酶切： 1. 切点将200μl tip头剪成吸口1.5mm左右的直径，MilliQ水涮洗一遍，吸100μl水后戳取斑点，将胶粒打入0.2ml EP管中，吸干水后再用MilliQ水洗涤两遍，吸干。 2. 脱色 180μl 50%MeOH/50mM NNH4HCO3脱色两次以上，每次半小时；180μl