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货号：DS3111-0032
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
nitrogen tank and thaw in a 37°C waterbath, shaking gently until the frozen aliquot is reduced to a small ice pellet. 6. Transfer cells into a 15-mL PP tube using a plastic transfer pipette and add ~10 mL of warm (37°C) wash medium drop
A quick RNA mini-prep for Neurospora mycelial cultures
pellet with 70% EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12. 15. Spin 10-20 min in microfuge. Wash twice with 70% EtOH and dry
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