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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温或冷藏
- 保质期:
1年或咨询
- 英文名:
Thermo Fisher PS 2.0UM 10% 100ML
- 库存:
999
- 供应商:
北京泽平
- CAS号:
不适用
- 规格:
100ml/瓶
赛默飞5000系列微粒悬浮液,2.0µm,10%固体,100ml Thermo Fisher PS 2.0UM 10% 100ML 货号:5200B



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文献和实验于4℃,期限1个月。 · 固体培养基 (1 liter ):称取21 g之Difco PPLO broth,0.02 g phenol red,15g Bacto agar于600ml蒸馏水中,加热溶解之。灭菌121℃,15分钟。放在50℃水中浴中,待温度降低至50℃时,于无菌操作台内加入200ml马血清,100ml之15% yeast extract solution 及100ml解冻之10x stock solution,混合均匀后,倒入60x50㎜之无菌培养皿中,5ml/培养皿。保存于4℃
Native Chromatin Preparation and Illumina/Solexa Library Construction
). 34. Resuspend the beads in 100 µL of 1X TE per tube. Add 3 µL of 10% SDS and 5 µL of 20 mg/mL proteinase K to each tube. Incubate overnight at 65°C. 35. Extract and precipitate the DNA as follows: i. Vortex
Isolation of Protein from Tissue
3ml Brij 96 10% 8.75ml NP40 10% 1.25ml Make up to 100ml with ddH2O. Protease inhibitors: Leupeptin 25µM Aprotinin 25µM AEBSF 25µM For 100ml Brij buffer add 100µl of at least two protease inhibitors. Reducing solution: Bromophenol blue
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