Sartorius赛多利斯VS0161Vivaspin 50

【资源】蛋白质浓缩，两个小tips

, a MWCO of 12,000 to 14,000 is appropriate, whereas a MWCO of 3500, 2000, or even 1000 is appropriate for peptides. Most dialysis membranes are made of derivatives of cellulose. They come in a wide variety of MWCO values, ranging from 500 to 500,000

Chromatin IP (CHIP assay)

PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction:      1 x 95 °C, 2 min      21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min      1 x 72

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl