赛默飞世尔Thermo Fisher DUAL COOLED

Two‐Hybrid Dual Bait System

.1 ) pLacGus (Fig. ) 90‐ or 100‐mm complete minimal (CM) medium dropout plates (unit 13.1 ) with 2% (w/v) glucose (Glu) or 2% (w/v) galactose (Gal) and 1% raffinose

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers

Preparation of Carboxypeptidase Y and Its Properties

of fresh bakers’ yeast is crumbled and mixed with 330 ml of chloroform. The mixture is occasionally kneaded with a spatula or glass rod, until the yeast has liquefied (30～60 min). When the cooled yeast has been delivered, the container of the mixture