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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310528052
中文名称:
英文名称:X50 Filters fluoropore membrane plain white 142mm dia 0.22µm pore size (pack of 50)
货期:热销品现货,其他请咨询




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文献和实验Southern Hybridization Protocols
8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65
min in 50 µl of buffer containing 100 mM sodium phosphate (pH 7.0), 5 mM EDTA, 1 mM NADPH, 1 mM NADH, 150 µM DTT, 5 mM ß-mercaptoethanol, 20 µM acetyl-CoA or n-octanoyl-CoA or any other acyl-CoA as needed, 4% DMSO, and 8 µM of ACP pretreated with DTT
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
of the 1000x tetracyclin stock solution . Inoculate with 25 to 50µl of the glycerol stock. Incubate in 37°C shaker incubator for 6-8 hours. Preparation of host cells from single colony Streak XL1-blue
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