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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_70310795683
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英文名称:X50 Microplate Corning 1536 wellassay standard plate flat bottom non treated, non-sterile polysty
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文献和实验Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
storage. PEG: 400 mls 50% PEG, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar
700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum
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