赛默飞世尔Thermo Fisher CENTRIVAP H
EXAGONAL ACID-RESIST ROTOR,1.5 ML TUBES 1,5/2,0 ml Reaktionsgefäße 货号：T_70310634843- ￥1200 - 24000
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货号：T_70310634843
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英文名称：CENTRIVAP HEXAGONAL ACID-RESIST ROTOR,1.5 ML TUBES 1,5/2,0 ml Reaktionsgefäße
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
Enrichment of PBMCs with monocytes (The Science Advisory Board)
1. Dilute buffy coat to 150 ml with PBS. 2. Prepare 2 Ficoll (Biocoll) gradients in Leucosep tubes (15 ml Ficoll per tube). 3. Apply 30 ml of diluted blood to each gradient. 4. Centrifuge 30 min at 400 x g without brakes. 5. Collect the PBMC fraction
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