赛默飞世尔Thermo Fisher X80 Micropl
ate Eppendorf sterile polypropylene (VE=80 Platten) 货号：T_70310723814- ￥1200 - 24000
- Thermo Fisher
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- T_70310723814
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310723814
中文名称：
英文名称：X80 Microplate Eppendorf sterile polypropylene (VE=80 Platten)
货期：热销品现货，其他请咨询
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文献和实验Cool growth competent E . coli
al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
(for plates) 2.SOC Media: SOB 20 mM Glucose 3.X-gal: Dissolve 100 mg of X-gal in 5 ml of dimethylformamide in a sterile polypropylene tube. Aliquot 1 ml into eppendorf tubes wrapped in foil (to prevent damage by light) and store at -20 degrees C.
Diatomaceous Earth-based Midi-prep
℃ with intermittent mixing. Spin at 8000 for 5min. Decant supernatant to Eppendorf tube. Spin again and transfer to Eppendorf tube (about 250 μl). Precipitate with 2.5 volumes of ethanol/acetate, rinse with 80% ethanol, and dry in a Speed-vac. Resuspend in 40 μl
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