赛默飞世尔Thermo Fisher NucleoSpin(
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货号：T_70312748432
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英文名称：NucleoSpin(R) 8 Plant II core kit, contains Plant II bindingstrips, buffers, RNase A, 48 x8 preps
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文献和实验【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
. Each primer in the pool also contains a single 5' nondegeneratenucleotide sequence derived from a codon consensus across the aligned aminoacid sequencesflanking the conserved motif. During the initial PCR amplification cycles, the degenerate core
RASL‐seq for Massively Parallel and Quantitative Analysis of Gene Expression
10 mM dNTPs NucleoSpin Extract II kit for PCR purification (Clontech, cat. no. 740609.50)
of saturation mutagenesis to identify and derive consensus sequence(s) for cis-acting-element(s). Identification of the minimal promoter. i & ii, the promoter is spliced to a reporter gene on a plasmid. iii nested deletions with exonuclease iii coupled
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