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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70315137823
中文名称:
英文名称:X1 HC32 Interchangeable block for 20x0.2ml microtubes plus 12x1.5ml microtubes
货期:热销品现货,其他请咨询




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文献和实验Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
off the nitrocellulose membranes and submerse in pyrex dish with 1 x PBS + 0.1% Triton X-100 (1 liter = 50ml 20x PBS + 979 ml dH2 O + 1ml Triton X-100). Wash with gentle shaking for 3 x 5 minutes at room temperature to remove agarose. Block with PBS + 2% BSA
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
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