赛默飞世尔Thermo Fisher Thermoblock

Karyotyping of X. tropicalis

for 20 minutes. 5. Pipette the dorsal halves, carrying as little water with them as possible, into an Eppendorf tube containing 0.2 ml of 60% acetic acid in water. 6. Allow to stand for 5 minutes. 7. Pipette all of the tissue, carrying

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

DNA Preparation from Adherent Cells

is precipitated. 8.Use a sterile glass pipette to transfer the precipitated DNA into a tube with 30 ml of 70% ethanol tube.Place on inverting rack and invert for 2 hr to thoroughly rinse.Transfer DNA into a sterile eppendorf tube. 9.Centrifuge for 20 min at 14