赛默飞世尔Thermo Fisher X50 Filters

Expression Library Screening (Procaryotic) Using AP-Fusion Proteins

off the nitrocellulose membranes and submerse in pyrex dish with 1 x PBS + 0.1% Triton X-100 (1 liter = 50ml 20x PBS + 979 ml dH2 O + 1ml Triton X-100). Wash with gentle shaking for 3 x 5 minutes at room temperature to remove agarose. Block with PBS + 2% BSA

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65

cDNA LIBRARY SCREENING

, the point is to neutralize, so there is no time limit 7. Transfer membranes to a 3MM paper presoaked with Wash solution and leave for 10 minutes 8. UV X-link. Place the membranes in a 3MM paper damp with wash solution