赛默飞世尔Thermo Fisher X960 Cryovi
al Cryobank 96 well format for protein/ cell storage uncoded 1mL sterile (supplied as 10 货号：T_70310309822- ￥1200 - 24000
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- 2025年07月12日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310309822
中文名称：
英文名称：X960 Cryovial Cryobank 96 well format for protein/ cell storage uncoded 1mL sterile (supplied as 10
货期：热销品现货，其他请咨询
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文献和实验overnight (12-16 hours) at 37degC with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen
and further incubate overnight (12-16 hours) at 37℃ with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen at -70℃
lysate to a fresh tube. Depending on the cell type you are using, this may or may not be necessary - however, it never hurts to do it. 7.) Determine protein concentration by whatever method you’re used to. 8.) Typically, I use 150-200μg
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