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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
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货号:T_70313111784
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英文名称:X600 Filter S-Pak 0.7µm gridded sterile, individua lly wrapped mixed cellulose esters 47mm for microb
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube
the pellet in 10 ml sterile 1 X TE/LiAC (made fresh from stocks of 10 X TE [0.1 M Tris-HCI, 0.01 M EDTA, pH 7.5] and 10X LiAC [1 M lithium acetate adjusted to pH 7.5 with dilute acetic acid]. Repeat. Pellet cells in microfuge at 7000rpm, discard sup
the pellet in 10 ml sterile 1 X TE/LiAC (made fresh from stocks of 10 X TE [0.1 M Tris-HCI, 0.01 M EDTA, pH 7.5] and 10X LiAC [1 M lithium acetate adjusted to pH 7.5 with dilute acetic acid]. Repeat. Pellet cells in microfuge at 7000rpm, discard sup
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