赛默飞世尔Thermo Fisher HPLC COLUMN

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

Nano-LC Coupled to MS/MS . In summary: i. Pull a fused-silica capillary microcolumn (100 µm I.D. x 365 µm O.D.) with a P-2000 laser puller. ii. Pack the microcolumn with 10 cm of C18 reversed-phase material

HPLC培训教程

效应。 2．柱的发展方向 因强调分析速度而发展出短柱，柱长3~10cm，填料粒径2~3µm。为提高分析灵敏度，与质谱(MS)联接，而发展出窄径柱、毛细管柱和内径小于0.2mm的微径柱（microbore）。细管径柱的优点是：①节省流动相；②灵敏度增加；③样品量少；④能使用长柱达到高分离度；⑤容易控制柱温；⑥易于实现LC-MS联用。 但由于柱体积越来越小，柱外效应的影响就更加显著，需要更小池体积的检测器（甚至采用柱上检测），更小死体积的柱接头和连接部件。配套使用的设备应具备如下性能

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using