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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310327282
中文名称:
英文名称:PIPETMAN CLASSIC P200 50-200µL channel, manual, air displacement variable volume
货期:热销品现货,其他请咨询




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文献和实验Marcantonio Lab Protocol Manual
. 3) Holding the plates at a 45 degree angle so that the gel will flow down one of the side spacers, carefully inject the gel between the glass plates using a 50 ml syringe, maintaining an even flow to avoid the formation of air bubbles. If air bubbles form
7. Resuspend the DNA pellet in 20 µ l of TE (pH8.0). Gently resuspend with finger-vortexing (to prevent shearing of the DNA DO NOT VORTEX). Place the tube in a 50 degrees C waterbath for 15 minutes to aid resuspension. 8. Run the DNA on a minigel
to the cell pellet in the order listed: Component Volume (µl) 1. PEG 3500 50% w/v 240 µl 2. LiAc 1.0 M 36 µl 3. Boiled SS-Carrier DNA (2 mg/ml) 50 µl 4. Plasmid DNA (0.1 to 1 µg) plus water 34 µl Total Volume 360 µl Be sure
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