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北京泽平
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货号:T_70310153593
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英文名称:X50 TRANSFER MEMBRANE IMMOBILON-PSQ APPLICATIONS include high-yield protein sequencing , low-molecu
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文献和实验Replication timing by density transfer
(frozen slanted to maximize the surface area). During the experiment, mix 20 ml of cells with 1/50 volume of 10% Na-azide in a 35 ml Corex tube (on ice) and immediately transfer the mix to a tube of frozen EDTA/azide. Alternatively, squirt the 10% azide
.g., skin tumors, and for gene therapy ( 2 , 3 , 4 ) . The various applications of MEP include, in addition to the direct functional transfer of genes (electrotransfection, electrogenetransfer) and drugs, the release of proteins
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
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