赛默飞世尔Thermo Fisher X1000 Tube

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, 1 mM ATP, 2 mM DTT, 1 mM MgCl2 , 2 mg/ml Tween-20, 7.5% (w/v) Triton X-100 and 0.2 mM PMSF) and stir gently at 30°C for 30 min. The extract was dialyzed against Buffer D (10 mM Tris-Cl, pH 8.0, 0.2 mM CaCl2 , 0.5 mM ATP, 0.1 mM dithiothreitol (DTT), 100

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), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

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. In addition, variations to the basic protocols and areas where special attentionis required for successful application of these methods are discussed. Key Words: Cell adhesion; integrins; extracellular matrix; fibronectin; N-acetyl-β-D-glucosaminidase