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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310568052
中文名称:
英文名称:X50 Culture plate insert Millicell-HATF inserts 12mm dia 0.45µm pore size (pack of 50)
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
) Embryonic culture dish Prepare by pouring 3 mL of agar substrate into a 35-mm Petri dish (Fisher Scientific, 0875711YZ) (Chapman et al. 2001 ). Embryonic culture insert Prepare by pouring 1 mL of agar substrate into a Millicell
(from R. Plattner & A.M. Pendergast) 1. Following stimulation, place cells on ice and wash 2X with cold PBS 2. Lyse each 100 mm plate with 500-1000 μl of lysis buffer (50mM HEPES pH7.0, 150 mM NaCl, 10% glycerol, 1% triton-X-100, 1.5 mM MgCl
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