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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310134051
中文名称:
英文名称:X200 Centrifuge tube filters NY Spin-X 500µL membrane nonpyrogenic
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
high background, but does not make the length of chromatin DNA short enough for ChIP. (10) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. Do not take yolk proteins precipitated in the bottom of the tube. (11) Add 55.6 µl of 10
Southern Hybridization Protocols
distilled water or TE in a microcentrifuge tube by heating for 5 min in a boiling water bath; then immediately cooled on ice. Perform the following adddtions on ice: 1 µ l 500 µ M dATP 1 µ l 500 µ M dGTP 1 µ l 500 µ M dTTP 20 µ l 2.5x Random
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube
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