赛默飞世尔Thermo Fisher X100 Multiw
ell plate Corning sterile certified non-pyrogenic polystyrene 24 well (15.6mm dia) 货号：T_70310377841- ￥1200 - 24000
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北京泽平
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货号：T_70310377841
中文名称：
英文名称：X100 Multiwell plate Corning sterile certified non-pyrogenic polystyrene 24 well (15.6mm dia)
货期：热销品现货，其他请咨询
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文献和实验Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
Large-scale yeast transformation大规模转化酵母【Yale University】
is that cells not be stationary. 2.If you are going to plate transformations, a larger pellet could be used - up to 10 ul? 3.Boehringer 146-7-140. We omitted this for months. It didn't seem to affect transformation efficiency at all, but maybe it affects non
---------------------------------------------------------------------------- Day 2 1. Heat a tube of carrier DNA in a boiling water bath for 5 min and then chill in ice/water. 2. Scrape a 50 ml blob of yeast from the YPAD plate and suspend the cells in 1 ml of sterile water in a 1.5 ml
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