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北京泽平
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EA
货号:T_70312333529
中文名称:
英文名称:Refractometer 0°C to -40°C (freezing point) antifreeze / battery acid Bellingham and Stanley,
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
of C. elegans embryos is attached to microscope slides, permeabilized by freezing and fixed with methanol and formaldehyde. Embryos are then incubated overnight with a digoxigenin-labelled single-stranded DNA probe, followed by extensive washes
), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Polyclonal and Monoclonal Antibodies
1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate
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