赛默飞世尔Thermo Fisher X50 SPE car

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

Solution: 100 mM NaCl; 5 mM MgCl2 ; 100 mM Tris (pH 9.5); 0.1% Tween 20; 1mM Levamisole. Levamisole is a potential inhibitor of endogenous phosphatases. Culture tubes: Available from Fisher as 6 mm (ID) x 50 mm (length) in either Pyrex or borosilcate

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column