赛默飞世尔Thermo Fisher X2850 28MM
CAP (FOR GLASS UNIVERSAL) FOR AUTOCLAVI NG POLYPROPYLENE (PACK OF 2850) 货号：T_70312692570- ￥1200 - 24000
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999
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北京泽平
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货号：T_70312692570
中文名称：
英文名称：X2850 28MM CAP (FOR GLASS UNIVERSAL) FOR AUTOCLAVI NG POLYPROPYLENE (PACK OF 2850)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Northern Blotting方法(Howell Lab)
Make a 10 ng/µl dilution of the probe. Add 10 µl (100 ng) of probe to a 0.5 ml screw-cap eppendorf tube. Adjust volume to 28 µl with water. (ie, 7 µl of probe needs 21 µl H2 O.) Boil for 5 min and then chill on ice for 5 min.
). Each extension reaction contained 500-750 ng of Biomek isolated single-stranded DNA, reaction buffer, nucleotide extension mix, oligonucleotide primer (typically M13 (-40) universal sequencing primer, see Appendix D), either [a-32-P]dATP or [a-35-S]dATP and Bst
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