赛默飞世尔Thermo Fisher Labelling r

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs

of individual representative nuclei. Materials & Reagents Buffer A Prepare as follows for 10 ml of buffer (make it fresh): 8.3ml - ddH2 O 0.6ml - KCl (1 M stock solution) (60mM

Southern Hybridization Protocols

-phosphate buffer pH 7.2 7% (w/v) SDS 2% (w/v) BSA 1 mM EDTA pH 8.0 Preparation of solution (not easy to prepare therefore follow the recipe):  Prepare 0.3 M Sodium-phosphate buffer by adding 40.2g NaH2PO4 to 400 ml sterile distilled water

RNA甲醛琼脂糖凝胶分离的Northern杂交

of ~ 1mm. Prepare sample: Adjust the volume of each RNA sample to 6 µl with water, then add 2.5 x 6 µl freshly prepared sample denaturation mix. Mix by vortexing, microcentrifuge briefly to collect liquid, and incubate 15 min at 55 oC. Cool on ice