赛默飞世尔Thermo Fisher X10 Membran
e filter AmiconBioseparations ultrafilt NMGG=3000, D=25mm (VE=10Stck.) 货号：T_70310440221- ￥1200 - 24000
- Thermo Fisher
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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310440221
中文名称：
英文名称：X10 Membrane filter AmiconBioseparations ultrafilt NMGG=3000, D=25mm (VE=10Stck.)
货期：热销品现货，其他请咨询
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文献和实验Northern Blotting方法(Howell Lab)
Fixing Bake membrane in 80℃ vacuum oven for 20 min. UV crosslink (on optimal setting of Fisher Crosslinker) the membrane (face side up). Store between 2 pieces of chromatography paper in a plastic bag. Labelling the probe
BP 152-5 (MW 121.14) (890 mM) 110g Boric acid Fisher (electroph.gr) BP 168-500 (MW 61.83) (20 mM) 40 mL 0.5M EDTA pH 8.0 H2O to 2L, check pH w. strip (take out and drizzle ~500 ul on pH strip, ~pH 8.5, filter sterilize 0.2
-stack from the bottom of the pseudopodia up through the filter and through the cell body), use polyester filters (e.g. CoStar Transwell Clear) rather than the more opaque polycarbonate. Although certainly not totally ‘clear’, the polyester membrane
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