赛默飞世尔Thermo Fisher X30 SPE col
umn HyperSep Aminopro pyl 1g bed weight 60A pore size aminopropyl 40µm 货号：T_70310045824- ￥1200 - 24000
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货号：T_70310045824
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英文名称：X30 SPE column HyperSep Aminopro pyl 1g bed weight 60A pore size aminopropyl 40µm
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文献和实验dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
activation by G‐protein‐coupled receptors. Nat. Rev. Mol. Cell. Biol. 9:60‐71. Popov, S., Yu, K., Kozasa, T., and Wilkie, T.M. 1997. The regulators of G protein signaling (RGS
the homogenate at 100,000 x g (29,000 rpm) for 60 min at 4oC in a 50.2Ti rotor to remove undisrupted tissue from cell cytosol. At room temperature, carefully collect the cytosolic supernatants from the tubes with a pipette, pool them in a graduated cylinder
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