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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310351930
中文名称:
英文名称:DATALOGGER SET T° HR% readings temperature & humidity C, F, %rF, % RH
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
C. elegans whole mount immunohistochemistry with Bouin's Fixative
) solution two times. Incubate 30 minutes or more then begin standard Ab incubations. I never switch tubes during this procedure. I store these worms at 4°C and they often stain relatively effectively a month later (and sometimes they don't). In my hands
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
sec. break x 3 times, 20% amplitude with a small tip). Now the length of your chromatin DNA should be 200-1000 bp. (13) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. This is the soluble sheared chromatin fraction. This can be stored
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