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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311783154
中文名称:
英文名称:X250 Container Fisherbrand snap cap non-sterile polypropylene non graduated 960mL snap cap separat
货期:热销品现货,其他请咨询




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文献和实验TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
with tissue adherence on slides during the hybridization procedure. Immerse tissue in sterile 15% sucrose/1xPBS (recipe follows) 3 hrs. to overnight at 4°C. Embed tissue in O.C.T. (Baxter No. M7148-4), M1 (Lipshaw) or any other convenient embedding
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
for up to two weeks. 15% Sucrose in PBS: 500ml sterile PBS 75g "RNase free" sucrose Mix above and filter sterilize with a disposable Nalgene filtration unit type S(0.45 micron). Store at 4°C. USE OF FISHERBRAND SUPERFROST
in a dry incubator. 9) Colonies should be present within 12h, but incubation can go for up to 24h. Alternatively, the plate can be kept on a clean bench top for ~48hrs (e.g. over a weekend). Note: Snap-cap Falcon tubes (e.g. 2059’s) can be used
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