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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310640771
中文名称:
英文名称:HiScreen HIC Butyl S Fast Flow column, 4.7mL Store at Room Temperature
货期:热销品现货,其他请咨询




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文献和实验and minicolumn. Slow flow of 1-2 drops per second is necessary for efficient binding of the DNA to column. III. Washing the Column Reload the syringe with 2-3 ml low salt buffer. Reconnect syringe to column and push low salt buffer through. Remove
) Resuspend in 1 ml TE buffer 5) Extract purified DNA with Phenol/Chloroform (Maniatis E.3) a) Add one volume phenol (pre-equilibrated with Tris buffer, pH 8.0) b) Centrifuge 10 minutes @10,000 RPM, room temperature.
). 7. Place the washed and drained column into a new eppendorf tube and add 50 'micro'l of preheated MQ water (70 - 80 °C) or TE buffer (at room temperature) to elute the DNA and place at room
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