赛默飞世尔Thermo Fisher Receiving f

Purification of Antiserum or Ascites by Protein A/G Chromatography

Agarose Affinity ColumnTypically, columns containing 5 ml or 10 ml of packed protein A/G Agarose are prepared. The size of the column is determined by the binding capacity of protein A/G and the amount of antiserum that must be processed. Protein

Agarose Gel Electrophoresis for the Separation of DNA Fragments

. The volume of the buffer should not be greater than 1/3 of the capacity of the flask.    2)      Add running buffer to the agarose-containing flask. Swirl to mix. The most common gel running buffers are TAE (40 mM Tris-acetate, 1 mM EDTA) and TBE (45 mM Tris-borate

Endothelial Cell Systems - Instructions For Use

the flask. For each 25 cm2 of cells to be subcultured, allow 3 ml of Clonetics® Trypsin/EDTA (T/E) to thaw and come to room temperature. For each 25 cm2 of cells to be subcultured, allow 5 ml of Clonetics® HEPES Buffered Saline Solution (HEPES-BSS