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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310458712
中文名称:
英文名称:X10 Filters Immobilon-P transfer membranes sheets 90 x 120mm 0.45µm pore size (pack of 10)
货期:热销品现货,其他请咨询




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文献和实验Two dimensional peptide mapping
so that you have at least 500 cpm. Transfer the protein to nitrocellulose, Immobilon or Nylon according to Kamps's Western Protocol . Tryptic digestion can be done with either NC, Nylon, or Immobilon. Nitrocellulose seems to give more reproducible yields of peptides.
Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
Kamps's Western Blotting Protocol
use Immobilon membranes for westerns.Although there are suggestions that nitrocellulose may give a lower background than Immoblion in ECL,most people prefer Immobilon even for ECL.If you are using Immobilon,be sure to wet it first in methanol briefly
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