赛默飞世尔Thermo Fisher X100 COMBIT

Loss of Heterozygosity

of the specific primer set. After PCR, remove the samples from the thermal cycler and dispense 2 µl of formamide/dye solution (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylenecyanole) into each reaction tube. Store reactions at 4°C until the gel

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

anddiluted further to 20 mM as working solutions (see Table 1). Store at -20°C. 12. Agarose powder. 13. Ethidium bromide at 10.0 mg/mL (Invitrogen). Table 1 Sequence (5‘→3') of oligonucleotides used in STA-PCR and SplinkBlunt-PCR  

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2