赛默飞世尔Thermo Fisher Membrane fi
lter type ME 24 diam. 47 mm plain mixed cellulose ester 47mm diameter (pack 货号：T_70310292131- ￥1200 - 24000
- Thermo Fisher
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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310292131
中文名称：
英文名称：Membrane filter type ME 24 diam. 47 mm plain mixed cellulose ester 47mm diameter (pack
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
3. Leave the wash at 65℃ for 30 min. Repeat.Take out the membrane and dry it on paper towel. Wrap with Saran film & place it in a film cassette. Monitor radioactivity of the membrane to estimate exposure times: >1000 counts: 2 hours; 200-300 counts, 24
Site-directed hydroxy radical mapping of nucleosome positions in vitro
filter devices are convenient for filtration. Xenopus laevis H2A wild type, H2B wild type, H3 Cys110Ala and H4 Ser47Cys as pET3 recombinant expression plasmids are available from Prof Tim Richmond, Dr Karolin Luger or myself. Back to top
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
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