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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310269554
中文名称:
英文名称:17MM PVDF Spritzenvorsatzfilter, ScientificTarget PVDF, medical grade polypropylene
货期:热销品现货,其他请咨询




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文献和实验of fatty acid synthesis inhibitors (refs. 7,8). Materials Reagents DTT was from Fisher (BP172-5); beta-mercaptoethanol was from Bio-Rad (161-0710). [2-14C]Malonyl-CoA (60 mCi/mmol, Perkin Elmer, NEC612), ACP (Sigma, A7303) was pretreated with 3 mM DTT
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Gel-elongation assay for type II fatty acid synthesis
Elmer, NEC612 ), ACP (Sigma, A7303 ) was pretreated with 3 mM DTT on ice for 20 min, aliquoted and stored at -80 °C. Cerulenin and thiolactomycin were purchased from Sigma. Triclosan could be obtained from VWR, 003384. Urea, PVDF membrane, 10X Tris
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