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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311789325
中文名称:
英文名称:X960 Pipette tip Corning Deckworks graduated, low binding barrier, sterile in hinged rack, rack
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
the homogenate at 100,000 x g (29,000 rpm) for 60 min at 4oC in a 50.2Ti rotor to remove undisrupted tissue from cell cytosol. At room temperature, carefully collect the cytosolic supernatants from the tubes with a pipette, pool them in a graduated cylinder
1 µl of air. 6. Load 2 µl of Loading Buffer from the reservoir. 7. Place tips in clean wells of disposable mixing tray and allow pipette to mix the sample and loading dye. 8. Place the pipette tip in a 50 well row so that the tip
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