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货号:T_70311373815
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英文名称:HEATING BLOCK CORNING SINGLE FOR 20 X 2.0ML TUBES for Corning LSE dry bath heater
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文献和实验> B. DNA PREPS FROM MANY INDIVIDUAL FLIES A similar method can be used with 96-well (8 x 12) micro plates to prepare DNA from a large number of individual flies. Up to 80 flies can be tested with a single plate. Place one anesthetized
(or room temp.) for 20-30 minutes. Inactivate the Proteinase K by heating to 95 C for 1-2 minutes. NOTES: This preparation can be stored at 4 C for months. We typically use 1 ul of the DNA prep in a 10-15 ul reaction
Single-molecule analysis of DNA replication by molecular combing
of complete synthetic medium. Add α-factor (1 µg/ml) for at least 2.5 hours to arrest cells in G1 . Add a second dose of α-factor part way through the incubation to ensure that cells do not escape the G1 block. Add BrdU to a final concentration
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