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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310238250
中文名称:
英文名称:X100 Filter disc Millipore nylon 25mm diameter 120µm mesh opening (pack of 100)
货期:热销品现货,其他请咨询




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文献和实验mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentr,hi) RPMI-1640 medium Tritn X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprtinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh the day f
Screening lambda cDNA or genomic libraries
was good this will be sufficient. Solutions : 5X OLB : (oligonucleotide labelling buffer) 250 mM Tris pH 8.0 250 µl 1M 25 mM MgCl2 25 µl 1M 5 mM ß-mercaptoethanol 0.35 µl 14.4 M stock 2 mM dCTP 20 µl 100 mM stock 2 mM dGTP 20 µl 100 mM stock 2 mM
on ice for 30 min (all antibodies 1/100 v/v). 4. Wash with 10 mL of PBS/FBS, spin and resuspend in PBS/FBS with 1 µg/mL of propidium iodide. Filter through 70 µm nylon mesh. 5. Select cells which are PI negative, c-kit positive, Sca-1 positive
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