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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310488742
中文名称:
英文名称:X25 PROTEIN CONCENTRATOR VIVASPIN 500 FOR SAMPLES 100 to 500µL polyethersulfone starter pack (5 of
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
dilution series of input DNA as standard curve templates. (500, 100, 20, 4 pg/µl) Duplicate PCR reactions for standard curve templates, and triplicate for test samples. PCR cycle settings Cycle 1: ( 1X) Step
Bicinchoninic Acid (BCA) Protein Assay (Smith)
: 4 gm BCA in 100 ml distilled water. 3.Reagent C: 0.4 gm cupric sulfate (5 x hydrated)in 10 ml water. 4.Working solution: Mix 1 volume reagent C with 25 volumes reagent B,then add 26 volumes reagent A to the C/B mixture. Assay 1.Prepare samples
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