赛默飞世尔Thermo Fisher X12 Filter

Procedures for In Vitro Production of Bovine Embryos

Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

) and place into the bottle of 37°C warmed PBS-glucose prepared previously to create collagenase solution. Mix well and sterile filter into a separate bottle. Place back into the 37°C water bath. Place the stop solution into the 37° C water bath as well.

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

tip) alternatively:       Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade);       9'' Pasteur pipettes;       70% Ethanol;       Alcohol burner or Bunsen burner;       Sterile plastic Petri dishes (100 x 15 mm);       Sterile