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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310174191
中文名称:
英文名称:X12 Filter system sterile CA membrane 0.45µm pore size 150mL membrane diameter 50mm (pack of 12)
货期:热销品现货,其他请咨询




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文献和实验Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)
module, all from Waters and a Rheodyne model 7125 injector with 20‐µl loop. The column was a Zorbax Bio‐Series GF‐250 with guard column (Dupont). The mobile phase was 0.2 M sodium phosphate buffer, pH 7.0, and the flow rate was 1 ml/min. This figure
albumin (Fraction V) Make up in PBST and sterile filter. Keep at 4°C to prevent bacterial contamination. 10xPBST 1L PH=7.4 Nacl 80g KCl 2g Na2HPO4 144g KH2PO4 0.24g Tween20 3ml 10xSecondary washing solution 1L PH=7.5 150mM NaCl 8.766g 50
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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