赛默飞世尔Thermo Fisher X12 Filter

Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)

module, all from Waters and a Rheodyne model 7125 injector with 20‐µl loop. The column was a Zorbax Bio‐Series GF‐250 with guard column (Dupont). The mobile phase was 0.2 M sodium phosphate buffer, pH 7.0, and the flow rate was 1 ml/min. This figure

原创：western protocol

albumin (Fraction V) Make up in PBST and sterile filter. Keep at 4°C to prevent bacterial contamination. 10xPBST 1L PH=7.4 Nacl 80g KCl 2g Na2HPO4 144g KH2PO4 0.24g Tween20 3ml 10xSecondary washing solution 1L PH=7.5 150mM NaCl 8.766g 50

v468 Chapter 2 Wnt活性蛋白分离及应用

dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH