赛默飞世尔Thermo Fisher X50 Syringe

Methods for DNA isolation

101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Preparation of Embryonic Extract

. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml