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北京泽平
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EA
货号:T_70311951861
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英文名称:X960 Pipette tip Corning Deckworks graduated, low binding, ext length, NS in Deckrack hinged rack,
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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
as stage X, nearly 24 h earlier in development than most electroporation protocols. Compared to in ovo electroporation, the ex ovo method allows easier embryo orientation (the posterior half of the embryo is darker than the anterior half
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
the homogenate at 100,000 x g (29,000 rpm) for 60 min at 4oC in a 50.2Ti rotor to remove undisrupted tissue from cell cytosol. At room temperature, carefully collect the cytosolic supernatants from the tubes with a pipette, pool them in a graduated cylinder
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